This study demonstrates that the Rotor-Gene 6000 thermal cycler is a reliable platform for use with the BD GeneOhm assay. The sensitivity of the BD GeneOhm assay was increased to 100% when the results of in-house PCR and patient note review were taken into account.
Sensitivities and specificities of 85.3% and 95.8% for the Rotor-Gene and 81% and 95.7% for the Smart-Cycler were obtained, compared with broth enrichment.
#Rotor gene 6000 thermal cycler software
By grouping samples into clusters, Rotor-Gene ScreenClust HRM Software opens a new dimension in HRM analysis for applications such as genotyping and mutation screening. A total of 1,428 pooled MRSA screens were tested. This software is the most powerful tool currently available for analysis of HRM data from the Rotor-Gene Q or Rotor-Gene 6000 cycler. Samples yielding discrepant results were subjected to detailed analysis with an in-house PCR and patient note review. Results were compared with the same assay performed on the Smart-Cycler II platform and overnight broth culture. In this study, one of the largest to date, we evaluated the performance of the BD GeneOhm MRSA assay on the Rotor-Gene 6000 thermal cycler, using samples taken directly from pooled MRSA screens. Bioz Stars score: 86/100, based on 1 PubMed citations. As health services move toward universal methicillin-resistant Staphylococcus aureus (MRSA) screening for hospital admissions, the most cost-effective approach is yet to be defined. Corbett Life Science rotor gene 6000 series 2 plex real time pcr thermal cycler Rotor Gene 6000 Series 2 Plex Real Time Pcr Thermal Cycler, supplied by Corbett Life Science, used in various techniques.
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